TurboKnockout® gene targeting service is founded on traditional embryonic stem cell (ESC)-mediated targeting techniques, which is often used for complex gene modeling projects to offer C57BL/6 or BALB/c mouse models with accurate genetic modification and no off-target effects.Cyagen custom knock out mouse Our TurboKnockout® service can provide you with conditional knockout, reporter knockin and humanization mouse models as fast as 6-8 months.
Cyagen's TurboKnockout® Gene Targeting Service
This proprietary gene targeting method is enabled by two innovations:
(1) A super competent ES cell line that generates 100% ESC-derived founder mice rather than chimeras, eliminating the need to screen for germline transmission.
(2) A selection cassette on the targeting construct capable of removing itself after ES cell targeting without the necessity to breed to Flp deleter mice.
These innovations eliminate two generations of breeding, shortening production time by 4-6 months as compared to industry standard. For conditional knockouts, it might be possible to breed founders straight to your tissue-specific Cre mice.
Advantages of TurboKnockout® Gene Targeting
Weighed against CRISPR/Cas9-based techniques, TurboKnockout® is free of patent disputes, provides precise models on a similar timeline, and may be the technology of choice among our clients performing drug development projects.
Freedom to Operate: Technology of choice for drug development projects
Success Rate: 100% guaranteed germline transmission (GLT)
Turnaround Time: Founders as fast as 6-8 months
Large Fragment Knockin: Insert fragments as much as 300 kb
Flexible Floxing: No size limitations for the floxed region
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