Cannabidiol (CBD) Oil

Cannabidiol (CBD) Oil Does Not Display an Entourage Effect in Reducing Cancer Cell Viability in vitro
Several studies have found that cannabinoids, especially delta-9-tetrahydrocannabinol and cannabidiol (CBD), have the potential to reduce cancer cell viability. The ongoing debate about the use of medical cannabis revolves around the effectiveness of pure plant versus intact plant material for treatment. Proponents for the use of intact plant material or botanical extracts argue that there is a synergistic effect between various cannabinoids, terpenoids, and flavonoids; This is commonly known as the "encouraging effect". Our study was designed to test the validity of the proposed entourage effect in a narrow application using a cancer cell viability model. Materials and methods: Six cancer cell lines from 3 different types of human cancers were treated with 10 μM pure CBD or 10 μM CBD from hemp (Cannabis sativa) oil, for 48 hours (from 3 different commercial sources Obtained) oil and cell viability was measured with the MTS assay. Dose-response curves were compared to compare the ability of pure CBD to CBD oils. CBD concentrations in commercial oils were independently confirmed, and cannabinoid and terpene composition was also compared. Results: CBD (10) M) was able to reduce cell viability in 3 of the 6 cell lines tested, and was found to be cell line-specific and not specific for cancer selection. None of the CBD oils tested was able to significantly reduce viability compared to pure CBD. Additionally, lower IC50 values were found for pure CBD than the most potent CBD oil in dose-response curves. Interestingly, some oils actually appeared to protect cancer cells from the effects of CBD. You can read about the cbd oil experience Conclusions: We found that pure CBD was as potent or more potent as the most potent oil test for reducing cancer cell viability, suggesting that there is no "entourage" effect under these specific in vitro conditions.
Method

1. cell cultures

Cell lines were obtained from the American Type Culture Collection and their identities were confirmed by short tandem repeat DNA profiling performed by Genetic Cell Line Testing (Burlington, NC, USA). As noted throughout, experiments were conducted in the presence of fetal bovine serum to antagonize serum-deprived cells to better mimic the pharmacokinetic conditions of the cancer cell environment in vivo.

2. Cell Treatment and Viability Assay

The CRC cell lines were treated as previously described, with cells preferred at a density of 10,000 cells/well [13]. Melanoma and GBM cells were maintained at a density of 7,500 cells/wells and treated 12 hours later with pure CBD (Cayman Chemical; Ann Arbor, MI, USA) or CBD oils with an equivalent concentration of CBD (among other lower levels). was done; Other components of the oil - eg, cannabinoids, terpenes, and flavonoids). In all treatments, DMSO was maintained at a constant 1%. The oil manufacturers have not been listed due to a lack of consent for disclosure. MTS (3- (4,5-dimethylthizol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H-tetrazolium), diocesan; Milpitas, CA, USA) the assay was used; To assess feasibility as previously described.
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